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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
11/07/2008 |
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Data da última atualização: |
11/07/2008 |
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Autoria: |
SILVA-WERNECK, J. O.; DE-SOUZA, M. T.; DIAS, J. M. C. de S.; RIBEIRO, B. M. |
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Título: |
Characterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the fall armyworm (Spodoptera frugiperda). |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Canadian Journal of Microbiology, Ottawa, v. 45, p. 464-471, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
A Brazilian strain of Bacillus thuringiensis subsp. kurstaki, designated S93, was analyzed regarding its cry gene and protein contents and activity against the fall armyworm (Spodoptera frugiperda, Smith 1797). Bioassays using lyophilized powders of S93 or HD-l and third instar larvae of S. frugiperda showed a 12.3-fold lower LCso for the S93 strain when compared with the standard HD-l strain. The spore-crystal mixture, analyzed by SDS-PAGE, showed two major polypeptides of 130 and 65 kDa, corresponding to Cryl and Cry2 toxins, respectively. Western blot analysis showed that these proteins were immunologically related to the CrylA protein from B. thuringiensis subsp. kurstaki HD-73. The polymerase chain reaction technique (PCR) using total DNA from the S93 strain and specific primers showed the presence of crylAa, crylAb, and crylAc genes, and a crylA-type gene was localized in a plasmid of about 44 MDa. A cry lAb gene was isolated from a S93 plasmid DNA library and completely sequenced. Computer analysis showed that the gene sequence (GenBank acession number AF059670) is identical to crylAbl and has 91.6 and 85.9% identity with crylAal and crylAcl genes, respectively. The deduced amino-acid sequence showed a high degree of similarity with the amino-acid sequences of the Cry lAb 1 (100%), CrylAal (93.8%), and CrylAcl (90.6%) proteins.
Une souche de Bacillus thuringiensis subsp. kurstaki provenant du Bresil et identifiee S93 a ete etudiee en regard du gene cry et du contenu en proteines et pour son activite contre Ie legionnaire d'automne (Spodoptera
frugiperda, Smith, 1797). Les bioessais utilisant des poudres lyophilisees de S93 ou de HD-l et des larves du troisieme age de S. frugiperda ont montre une LCso 12.3 fois plus basse pour la souche S93 comparativement a la souche standard HD-1. Le melange spores-cristaux, analyse par SDS-PAGE, a revele deux polypeptides principaux de 130 et 65 kDa, correspondant aux toxines Cry 1 et Cry2 respectivement. Le buvardage Western a confirme que ces proteines etaient immunologiquement apparentees ala proteine CrylA de B. thuringiensis subsp. kurstaki HD-73. La reaction en chaine de la polymerase (PCR) utilisant l' ADN total de la souche S93 et des amorces specifiques a confirme la presence des genes cryJAa, cryJAb, et cryJAc et a permis de reperer un gene de type cryJA sur un plasmide ca. 44 Mda. Un gene cryJAb a ete isole d'une banque d'ADN plasmidique S93 et iI a ete completement sequence. Une analyse informatisee a montre que la sequence de ce gene (code d'identification GenBank AF059670) etait identique a celle de cryJAbJ et qu'elle etait a 91.6% et 85.9% identique a celle des genes cryJAaJ et cryJAcl respectivement. La sequence obtenue des acides amines a revele un degre eleve de similitude avec les sequences des proteines CrylAbl (100%), CrylAal (93.8%),35 CrylAc1 (90.6%). MenosA Brazilian strain of Bacillus thuringiensis subsp. kurstaki, designated S93, was analyzed regarding its cry gene and protein contents and activity against the fall armyworm (Spodoptera frugiperda, Smith 1797). Bioassays using lyophilized powders of S93 or HD-l and third instar larvae of S. frugiperda showed a 12.3-fold lower LCso for the S93 strain when compared with the standard HD-l strain. The spore-crystal mixture, analyzed by SDS-PAGE, showed two major polypeptides of 130 and 65 kDa, corresponding to Cryl and Cry2 toxins, respectively. Western blot analysis showed that these proteins were immunologically related to the CrylA protein from B. thuringiensis subsp. kurstaki HD-73. The polymerase chain reaction technique (PCR) using total DNA from the S93 strain and specific primers showed the presence of crylAa, crylAb, and crylAc genes, and a crylA-type gene was localized in a plasmid of about 44 MDa. A cry lAb gene was isolated from a S93 plasmid DNA library and completely sequenced. Computer analysis showed that the gene sequence (GenBank acession number AF059670) is identical to crylAbl and has 91.6 and 85.9% identity with crylAal and crylAcl genes, respectively. The deduced amino-acid sequence showed a high degree of similarity with the amino-acid sequences of the Cry lAb 1 (100%), CrylAal (93.8%), and CrylAcl (90.6%) proteins.
Une souche de Bacillus thuringiensis subsp. kurstaki provenant du Bresil et identifiee S93 a ete etudiee en regard du gene cry et du contenu ... Mostrar Tudo |
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Thesagro: |
Bacillus Thuringiensis; Controle Biológico; Spodoptera Frugiperda. |
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Categoria do assunto: |
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Marc: |
LEADER 03508naa a2200193 a 4500
001 1629824
005 2008-07-11
008 1999 bl --- 0-- u #d
100 1 $aSILVA-WERNECK, J. O.
245 $aCharacterization of Bacillus thuringiensis subsp. kurstaki strain S93 effective against the fall armyworm (Spodoptera frugiperda).
260 $c1999
520 $aA Brazilian strain of Bacillus thuringiensis subsp. kurstaki, designated S93, was analyzed regarding its cry gene and protein contents and activity against the fall armyworm (Spodoptera frugiperda, Smith 1797). Bioassays using lyophilized powders of S93 or HD-l and third instar larvae of S. frugiperda showed a 12.3-fold lower LCso for the S93 strain when compared with the standard HD-l strain. The spore-crystal mixture, analyzed by SDS-PAGE, showed two major polypeptides of 130 and 65 kDa, corresponding to Cryl and Cry2 toxins, respectively. Western blot analysis showed that these proteins were immunologically related to the CrylA protein from B. thuringiensis subsp. kurstaki HD-73. The polymerase chain reaction technique (PCR) using total DNA from the S93 strain and specific primers showed the presence of crylAa, crylAb, and crylAc genes, and a crylA-type gene was localized in a plasmid of about 44 MDa. A cry lAb gene was isolated from a S93 plasmid DNA library and completely sequenced. Computer analysis showed that the gene sequence (GenBank acession number AF059670) is identical to crylAbl and has 91.6 and 85.9% identity with crylAal and crylAcl genes, respectively. The deduced amino-acid sequence showed a high degree of similarity with the amino-acid sequences of the Cry lAb 1 (100%), CrylAal (93.8%), and CrylAcl (90.6%) proteins. Une souche de Bacillus thuringiensis subsp. kurstaki provenant du Bresil et identifiee S93 a ete etudiee en regard du gene cry et du contenu en proteines et pour son activite contre Ie legionnaire d'automne (Spodoptera frugiperda, Smith, 1797). Les bioessais utilisant des poudres lyophilisees de S93 ou de HD-l et des larves du troisieme age de S. frugiperda ont montre une LCso 12.3 fois plus basse pour la souche S93 comparativement a la souche standard HD-1. Le melange spores-cristaux, analyse par SDS-PAGE, a revele deux polypeptides principaux de 130 et 65 kDa, correspondant aux toxines Cry 1 et Cry2 respectivement. Le buvardage Western a confirme que ces proteines etaient immunologiquement apparentees ala proteine CrylA de B. thuringiensis subsp. kurstaki HD-73. La reaction en chaine de la polymerase (PCR) utilisant l' ADN total de la souche S93 et des amorces specifiques a confirme la presence des genes cryJAa, cryJAb, et cryJAc et a permis de reperer un gene de type cryJA sur un plasmide ca. 44 Mda. Un gene cryJAb a ete isole d'une banque d'ADN plasmidique S93 et iI a ete completement sequence. Une analyse informatisee a montre que la sequence de ce gene (code d'identification GenBank AF059670) etait identique a celle de cryJAbJ et qu'elle etait a 91.6% et 85.9% identique a celle des genes cryJAaJ et cryJAcl respectivement. La sequence obtenue des acides amines a revele un degre eleve de similitude avec les sequences des proteines CrylAbl (100%), CrylAal (93.8%),35 CrylAc1 (90.6%).
650 $aBacillus Thuringiensis
650 $aControle Biológico
650 $aSpodoptera Frugiperda
700 1 $aDE-SOUZA, M. T.
700 1 $aDIAS, J. M. C. de S.
700 1 $aRIBEIRO, B. M.
773 $tCanadian Journal of Microbiology, Ottawa$gv. 45, p. 464-471, 1999.
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Embrapa Agrobiologia (CNPAB) |
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